Karolina Šantl Zupan began her professional career with the Slovenian Philharmonic Orchestra. She has also worked as a solo flutist in the orchestra of the Slovene Opera and Ballet Ljubljana and still, from time to time, takes part in their productions. Her success as a teacher has been con honoured by her numerous students who have received prizes in state and International competitions, while also regularly performing at solo recitals and as soloists with various orchestras. She frequently takes part in summer schools, holding seminars and master classes.

Karolina Šantl Zupan has performed in various chamber ensembles with recognised Slovenian and foreign artists and is currently a member of the International flute quartet 4Syrinx. She has recorded for Slovenian radio and television and for RAI in Italy. As a soloist she performed with Slovenian Philharmonic Orchestra, RTV Slovenia and Freiburg symphonic orchestra. In her discography we find nine compact discs with works for flute and piano, flute and harp, and for flute quartet. She currently holds position as a full professor at both Ljubljana Academy of Music and Zagreb Academy of Music.